| First Author | Khuda SE | Year | 2004 |
| Journal | J Biol Chem | Volume | 279 |
| Issue | 44 | Pages | 46182-90 |
| PubMed ID | 15322101 | Mgi Jnum | J:94487 |
| Mgi Id | MGI:3512866 | Doi | 10.1074/jbc.M405347200 |
| Citation | Khuda SE, et al. (2004) The Sac3 homologue shd1 is involved in mitotic progression in mammalian cells. J Biol Chem 279(44):46182-90 |
| abstractText | Saccharomyces Sac3 required for actin assembly was shown to be involved in DNA replication. Here, we studied the function of a mammalian homologue SHD1 in cell cycle progression. SHD1 is localized on centrosomes at interphase and at spindle poles and mitotic spindles, similar to alpha-tubulin, at M phase. RNA interference suppression of endogenous shd1 caused defects in centrosome duplication and spindle formation displaying cells with a single apparent centrosome and down-regulated Mad2 expression, generating increased micronuclei. Conversely, increased expression of SHD1 by DNA transfection with shd1-green fluorescent protein (gfp) vector for a fusion protein of SHD1 and GFP caused abnormalities in centrosome duplication displaying cells with multiple centrosomes and deregulated spindle assembly with up-regulated Mad2 expression until anaphase, generating polyploidy cells. These results demonstrated that shd1 is involved in cell cycle progression, in particular centrosome duplication and a spindle assembly checkpoint function. |
