|  Help  |  About  |  Contact Us

Publication : Subdomain VIII is a specificity-determining region in MEKK1.

First Author  Tu Z Year  2003
Journal  J Biol Chem Volume  278
Issue  48 Pages  48498-505
PubMed ID  14500727 Mgi Jnum  J:114128
Mgi Id  MGI:3688364 Doi  10.1074/jbc.M304234200
Citation  Tu Z, et al. (2003) Subdomain VIII is a specificity-determining region in MEKK1. J Biol Chem 278(48):48498-505
abstractText  MAPK/ERK kinase kinase 1 (MEKK1) is a mitogenactivated protein kinase kinase kinase (MAP3K) of the stress-induced JNK pathway. Once activated, MEKK1 phosphorylates the MAP2K MKK4, which in turn phosphorylates JNK. MEKK1 also has the capacity to activate IKK, the central protein kinase of the NF-kappa B pathway. The molecular determinants responsible for the ability of MEKK1 to recognize specific substrates are poorly understood. We report here that select point mutations in subdomain VIII of the protein kinase domain of MEKK1 (MEKK1 Delta) differentially affect its ability to activate MKK4 and IKK, and consequently AP1 and NF-kappa B reporter genes. Moreover, binding of MKK4 to MEKK1 Delta protects the latter from cleavage at an engineered protease target site in subdomain VIII. Collectively these results provide evidence that subdomain VIII of MEKK1 is involved not only in binding to, but also in discrimination of, protein substrates.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

2 Authors

4 Bio Entities

Trail: Publication

0 Expression