| First Author | Parikh H | Year | 1995 |
| Journal | Gene | Volume | 154 |
| Issue | 2 | Pages | 237-42 |
| PubMed ID | 7890170 | Mgi Jnum | J:23968 |
| Mgi Id | MGI:71762 | Doi | 10.1016/0378-1119(94)00850-r |
| Citation | Parikh H, et al. (1995) Organization, sequence and regulation of expression of the murine Hoxa-7 gene. Gene 154(2):237-42 |
| abstractText | The genomic sequence of Hoxa-7 (encoding the HOXa-7 homeobox protein), including the coding region (0.7 kb), flanked by a 5'-upstream region (2.8 kb), a 3'-downstream region (1 kb) and interrupted by an intron (995 bp), was determined. Northern blot analysis indicated the transcript size of Hoxa-7 to be 2.1-2.4 kb. Reverse transcription-PCR and primer extension analysis established the 5'-boundary of the mRNA to be in the region 1166 nt upstream from the start codon. Transient transfection of various Hoxa-7::cat constructs in NIH 3T3 cells was used to characterize the transcriptional activity of the 5'-flanking region of the gene. Constructs containing 544, 274 and 71 bp of the region upstream from the transcription start point (tsp) exhibited 78, 203 and 407%, respectively, of the activity shown by a control construct containing 739 bp of the upstream region. These data suggested the presence of negative regulatory elements in the region from 544 to 71 bp upstream from the tsp. |
