| First Author | Bruno MA | Year | 1995 |
| Journal | Am J Physiol | Volume | 268 |
| Issue | 3 Pt 1 | Pages | L381-9 |
| PubMed ID | 7900819 | Mgi Jnum | J:23935 |
| Mgi Id | MGI:71690 | Doi | 10.1152/ajplung.1995.268.3.L381 |
| Citation | Bruno MA, et al. (1995) Structure and function of the mouse surfactant protein B gene [published errata appear in Am J Physiol 1995 Jun;268(6 Pt 1):section L following table of contents and 1995 Jun;268(6 Pt 3):section L 2 p following table of contents]. Am J Physiol 268(3 Pt 1):L381-9 |
| abstractText | The mouse surfactant protein B (SP-B) gene was isolated from a DBA/2J EMBL3 genomic library, and the nucleotide sequence and transcriptional start site was determined. The SP-B gene was comprised of 11 exons and 10 introns and spanned 9,882 bases. Repetitive elements of the B1, B2, and R families and several other motifs were located within nontranslated regions of the gene. The 5' flanking region of the gene was utilized to express a bacterial chloramphenicol acetyl transferase reporter gene in mouse and human pulmonary adenocarcinoma cell lines. Transcriptionally active regions of the promoter were identified that determined lung epithelial cell-specific gene expression. Regions of the gene promoter shared between the mouse and human SP-B gene were used to locate putative transcription factor binding sites for thyroid transcription factor-1 and hepatocyte nuclear factor-3. The 5' flanking region of the murine SP-B contains cell-specific cis-active elements that regulate SP-B expression in the respiratory epithelium. |
