| First Author | Singh SK | Year | 2013 |
| Journal | Nat Commun | Volume | 4 |
| Pages | 1830 | PubMed ID | 23652018 |
| Mgi Jnum | J:205742 | Mgi Id | MGI:5546313 |
| Doi | 10.1038/ncomms2823 | Citation | Singh SK, et al. (2013) GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy. Nat Commun 4:1830 |
| abstractText | Somatic hypermutation in B cells is initiated by activation-induced cytidine deaminase-catalyzed C-->U deamination at immunoglobulin variable regions. Here we investigate the role of the germinal centre-associated nuclear protein (GANP) in enhancing the access of activation-induced cytidine deaminase (AID) to immunoglobulin variable regions. We show that the nuclear export factor GANP is involved in chromatin modification at rearranged immunoglobulin variable loci, and its activity requires a histone acetyltransferase domain. GANP interacts with the transcription stalling protein Spt5 and facilitates RNA Pol-II recruitment to immunoglobulin variable regions. Germinal centre B cells from ganp-transgenic mice showed a higher AID occupancy at the immunoglobulin variable region, whereas B cells from conditional ganp-knockout mice exhibit a lower AID accessibility. These findings suggest that GANP-mediated chromatin modification promotes transcription complex recruitment and positioning at immunoglobulin variable loci to favour AID targeting. |
