| First Author | Kimura F | Year | 2003 |
| Journal | J Biol Chem | Volume | 278 |
| Issue | 27 | Pages | 25046-54 |
| PubMed ID | 12692137 | Mgi Jnum | J:84377 |
| Mgi Id | MGI:2667505 | Doi | 10.1074/jbc.M303438200 |
| Citation | Kimura F, et al. (2003) Cloning and characterization of a novel RING-B-box-coiled-coil protein with apoptotic function. J Biol Chem 278(27):25046-54 |
| abstractText | We have identified a novel RING-B-box-coiled-coil (RBCC) protein (MAIR for macrophage-derived apoptosis-inducing RBCC protein) that consists of an N-terminal RING finger, followed by a B-box zinc finger, a coiled-coil domain, and a B30.2 domain. MAIR mRNA was expressed widely in mouse tissues and was induced by macrophage colony-stimulating factor in murine peritoneal and bone marrow macrophages. MAIR protein initially showed a granular distribution predominantly in the cytoplasm. The addition of zinc to transfectants containing MAIR cDNA as part of a heavy metal-inducible vector caused apoptosis of the cells characterized by cell fragmentation; a reduction in mitochondrial membrane potential; activation of caspase-7, -8, and -9, but not caspase-3; and DNA degradation. We also found that the RING finger and coiled-coil domains were required for MAIR activity by analysis with deletion mutants. |
