| First Author | Magherini F | Year | 1998 |
| Journal | FEBS Lett | Volume | 437 |
| Issue | 3 | Pages | 263-6 |
| PubMed ID | 9824304 | Mgi Jnum | J:50526 |
| Mgi Id | MGI:1306903 | Doi | 10.1016/s0014-5793(98)01241-1 |
| Citation | Magherini F, et al. (1998) Cloning of murine low molecular weight phosphotyrosine protein phosphatase cDNA: identification of a new isoform. FEBS Lett 437(3):263-6 |
| abstractText | The low molecular weight phosphotyrosine protein phosphatase (LMW-PTP) is a 18 kDa cytosolic enzyme, involved in the negative regulation of cell proliferation. In different mammalian species LMW-PTPs are expressed in two molecular forms produced from a single primary transcript through an alternative splicing mechanism. In this paper we report the cloning, expression and characterization of mouse isoforms of LMW-PTPs (called m-IF1 and m-IF2), very similar to the corresponding rat and human isoenzymes. Moreover we have identified a third cDNA encoding a protein (m-IF2P) that presents three substitutions compared to m-IF2. This new isoform is still active on pNPP, although to a lower extent: this reduction is mainly due to the leucine to proline substitution in position 13, within the catalytic loop. The mRNA expression level of this isoform is comparable to those of m-IF1 and m-IF2. It is likely that a gene duplication process followed by mutations has generated this new gene. |
