First Author | Iguchi N | Year | 1999 |
Journal | FEBS Lett | Volume | 456 |
Issue | 2 | Pages | 315-21 |
PubMed ID | 10456331 | Mgi Jnum | J:56926 |
Mgi Id | MGI:1342904 | Doi | 10.1016/s0014-5793(99)00967-9 |
Citation | Iguchi N, et al. (1999) Molecular cloning of haploid germ cell-specific tektin cDNA and analysis of the protein in mouse testis. FEBS Lett 456(2):315-21 |
abstractText | Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules. We report here the molecular cloning of a new member of the tektin family, tektin-t, identified from a mouse haploid germ cell-specific cDNA library. Tektin-t mRNA encodes a protein of 430 deduced amino acids possessing RSNVELCRD, the conserved sequence of tektin family proteins. Western blotting showed a single band having a molecular weight of 86 kDa in the mouse testis. Immunohistochemistry of the testis showed that tektin-t is localized in the flagella of elongating spermatids from developmental step 15 to maturity. |