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Publication : Molecular cloning of haploid germ cell-specific tektin cDNA and analysis of the protein in mouse testis.

First Author  Iguchi N Year  1999
Journal  FEBS Lett Volume  456
Issue  2 Pages  315-21
PubMed ID  10456331 Mgi Jnum  J:56926
Mgi Id  MGI:1342904 Doi  10.1016/s0014-5793(99)00967-9
Citation  Iguchi N, et al. (1999) Molecular cloning of haploid germ cell-specific tektin cDNA and analysis of the protein in mouse testis. FEBS Lett 456(2):315-21
abstractText  Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules. We report here the molecular cloning of a new member of the tektin family, tektin-t, identified from a mouse haploid germ cell-specific cDNA library. Tektin-t mRNA encodes a protein of 430 deduced amino acids possessing RSNVELCRD, the conserved sequence of tektin family proteins. Western blotting showed a single band having a molecular weight of 86 kDa in the mouse testis. Immunohistochemistry of the testis showed that tektin-t is localized in the flagella of elongating spermatids from developmental step 15 to maturity.
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