| First Author | Graw J | Year | 1997 |
| Journal | Mutat Res | Volume | 382 |
| Issue | 1-2 | Pages | 75-8 |
| PubMed ID | 9360640 | Mgi Jnum | J:43966 |
| Mgi Id | MGI:1099217 | Doi | 10.1016/s1383-5726(97)00005-8 |
| Citation | Graw J, et al. (1997) Detection of a point mutation (A to G) in exon 5 of the murine Mgf gene defines a novel allele at the Steel locus with a weak phenotype. Mutat Res 382(1-2):75-8 |
| abstractText | A new mutation at the locus encoding the mast cell growth factor (Mgf) is described and designated as MgfSl-3Neu. Homozygous mutants have a light grey fur, sometimes with white patches. Homozygotes are fertile, but with reduced litter size, when mated inter se. Analysis of haematological parameters indicated no difference between mutant and wild-type mice. Sequence analysis of the cDNA obtained from the brain of homozygous mutants revealed an A-->G exchange at position 400 leading to a predicted amino acid exchange from Asn-->Leu at position 122. As a consequence of the predicted amino acid exchange an extension of the alpha-helical context and a decreased hydropathicity of the region at positions 101-125 can be deduced. This single amino acid exchange is outside of the known important domains of MGF and explains the weak phenotype of MgfSl-3Neu. |
