| First Author | Zgombić-Knight M | Year | 1997 |
| Journal | Genomics | Volume | 41 |
| Issue | 1 | Pages | 105-9 |
| PubMed ID | 9126489 | Mgi Jnum | J:40088 |
| Mgi Id | MGI:87426 | Doi | 10.1006/geno.1997.4637 |
| Citation | Zgombic-Knight M, et al. (1997) Gene structure and promoter for Adh3 encoding mouse class IV alcohol dehydrogenase (retinol dehydrogenase). Genomics 41(1):105-9 |
| abstractText | Class IV alcohol dehydrogenase (ADH) has been shown to function in vitro as a retinol dehydrogenase catalyzing the synthesis of retinoic acid, a pleiotropic gene regulator. To enable genetic studies on the function of this enzyme and regulation of its gene, we have screened a genomic library and isolated the mouse class IV ADH gene (Adh3). The complete mouse class IV ADH coding region was found in nine exons spanning a 14-kb region. Primer extension analysis was used to map the transcription initiation site to a position lying 30 bp upstream of the ATG translation start codon. Nucleotide sequence analysis of the promoter region indicated an absence of both TATA-box and GC-box sequences; this distinguishes it from the promoters for class I, II, and III ADH genes. Sequence comparison of the mouse and human class IV ADH promoters indicated that they share a conserved region located 125-145 bp upstream of the coding region containing adjacent sequences matching the consensus binding sites for transcription factors AP-1 and C/EBP. |
