| First Author | Methner A | Year | 1997 |
| Journal | Biochem Biophys Res Commun | Volume | 233 |
| Issue | 2 | Pages | 336-42 |
| PubMed ID | 9144535 | Mgi Jnum | J:39842 |
| Mgi Id | MGI:87191 | Doi | 10.1006/bbrc.1997.6455 |
| Citation | Methner A, et al. (1997) A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development. Biochem Biophys Res Commun 233(2):336-42 |
| abstractText | A novel G protein-coupled receptor was isolated from a cDNA derived from the cell line NH15-CA2 and a cDNA library from adult mouse brain using a PCR cloning strategy. The amino acid sequence of the candidate receptor DEZ showed homology to neuropeptide and chemoattractant receptors. Highest overall homology was found with the orphan receptor GPR-1 (65%), the angiotensin II receptor (62%), and the C5a anaphylatoxin receptor (60%). Northern blot analysis of dez revealed a predominant 2.6 kb mRNA species in NH15-CA2 cells. In situ hybridization experiments showed that dez is differentially regulated during development, with a prominent expression in developing osseous and cartilaginous tissue. It was also detectable in the adult parathyroid glands, hinting at a possible function in bone metabolism. |
