| First Author | Hemmerich S | Year | 2001 |
| Journal | Immunity | Volume | 15 |
| Issue | 2 | Pages | 237-47 |
| PubMed ID | 11520459 | Mgi Jnum | J:86759 |
| Mgi Id | MGI:2682055 | Doi | 10.1016/s1074-7613(01)00188-1 |
| Citation | Hemmerich S, et al. (2001) Sulfation of L-selectin ligands by an HEV-restricted sulfotransferase regulates lymphocyte homing to lymph nodes. Immunity 15(2):237-47 |
| abstractText | Lymphocytes home to lymph nodes, using L-selectin to bind specific ligands on high endothelial venules (HEV). In vitro studies implicate GlcNAc-6-sulfate as an essential posttranslational modification for ligand activity. Here, we show that genetic deletion of HEC-GlcNAc6ST, a sulfotransferase that is highly restricted to HEV, results in the loss of the binding of recombinant L-selectin to the luminal aspect of HEV, elimination of lymphocyte binding in vitro, and markedly reduced in vivo homing. Reactivity with MECA 79, an adhesion-blocking mAb that stains HEV in lymph nodes and vessels in chronic inflammatory sites, is also lost from the luminal aspects of HEV. These results establish a critical role for HEC-GlcNAc6ST in lymphocyte trafficking and suggest it as an important therapeutic target. |
