| First Author | Ukarapong S | Year | 2012 |
| Journal | Exp Cell Res | Volume | 318 |
| Issue | 5 | Pages | 662-9 |
| PubMed ID | 22285131 | Mgi Jnum | J:182299 |
| Mgi Id | MGI:5315196 | Doi | 10.1016/j.yexcr.2012.01.009 |
| Citation | Ukarapong S, et al. (2012) Megakaryocyte development is normal in mice with targeted disruption of Tescalcin. Exp Cell Res 318(5):662-9 |
| abstractText | BACKGROUND: Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H+ exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34+ hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation. OBJECTIVE: To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis. METHODS: We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development. RESULTS: Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal. CONCLUSION: Tescalcin does not appear to be necessary for normal megakaryocyte development. |
