| First Author | Dudley K | Year | 1991 |
| Journal | Genet Res | Volume | 57 |
| Issue | 2 | Pages | 147-52 |
| PubMed ID | 2055458 | Mgi Jnum | J:11281 |
| Mgi Id | MGI:59720 | Doi | 10.1017/s0016672300029220 |
| Citation | Dudley K, et al. (1991) Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x. Genet Res 57(2):147-52 |
| abstractText | The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3' portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1. |
