| First Author | Sridharan H | Year | 2017 |
| Journal | EMBO Rep | Volume | 18 |
| Issue | 8 | Pages | 1429-1441 |
| PubMed ID | 28607035 | Mgi Jnum | J:289444 |
| Mgi Id | MGI:5927398 | Doi | 10.15252/embr.201743947 |
| Citation | Sridharan H, et al. (2017) Murine cytomegalovirus IE3-dependent transcription is required for DAI/ZBP1-mediated necroptosis. EMBO Rep 18(8):1429-1441 |
| abstractText | DNA-dependent activator of interferon regulatory factors/Z-DNA binding protein 1 (DAI/ZBP1) is a crucial sensor of necroptotic cell death induced by murine cytomegalovirus (MCMV) in its natural host. Here, we show that viral capsid transport to the nucleus and subsequent viral IE3-dependent early transcription are required for necroptosis. Necroptosis induction does not depend on input virion DNA or newly synthesized viral DNA A putative RNA-binding domain of DAI/ZBP1, Zalpha2, is required to sense virus and trigger necroptosis. Thus, MCMV IE3-dependent transcription from the viral genome plays a crucial role in activating DAI/ZBP1-dependent necroptosis. This implicates RNA transcripts generated by a large double-stranded DNA virus as a biologically relevant ligand for DAI/ZBP1 during natural viral infection. |
