| First Author | Murakami M | Year | 2003 |
| Journal | Biochem Biophys Res Commun | Volume | 307 |
| Issue | 3 | Pages | 522-8 |
| PubMed ID | 12893253 | Mgi Jnum | J:84948 |
| Mgi Id | MGI:2670842 | Doi | 10.1016/s0006-291x(03)01186-0 |
| Citation | Murakami M, et al. (2003) Identification and characterization of the murine TRPM4 channel. Biochem Biophys Res Commun 307(3):522-8 |
| abstractText | The transient receptor potential (TRP) channels form a superfamily with six transmembrane structures, which is common in other types of voltage-dependent channels. The TRP-melastatin (TRPM) subfamily includes the putative tumor-suppressor melastatin, which was originally found as a down-regulated protein in melanoma tumor cell lines. Here, we report a novel TRP-related protein that is a murine orthologue of human TRPM4. The function of the novel murine TRPM4 was studied in HEK-293 cells using a fluorescent calcium indicator, fura-2. The removal and re-introduction of extracellular calcium triggered changes in the intracellular calcium only in cells expressing TRPM4a, which suggests that this novel channel plays a role in the calcium entry process. We also isolated a splice variant of TRPM4 that was proven to be non-functional. Both TRPM4 variants integrated into the plasma membrane. Furthermore, FRET analysis revealed that TRPM4a and TRPM4b localized close together, suggesting a multimerization of the two molecules. |
