| First Author | Adra CN | Year | 1987 |
| Journal | Gene | Volume | 60 |
| Issue | 1 | Pages | 65-74 |
| PubMed ID | 3440520 | Mgi Jnum | J:20057 |
| Mgi Id | MGI:68170 | Doi | 10.1016/0378-1119(87)90214-9 |
| Citation | Adra CN, et al. (1987) Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter. Gene 60(1):65-74 |
| abstractText | We report the cloning of the mouse pgk-1 gene encoding the somatic cell isoform of the enzyme phosphoglycerate kinase. The gene is contained within a 16-kb region of the X chromosome and is interrupted by at least ten introns. The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, the potential binding site for the Sp 1 transcription factor, a CCAAT sequence, but no TATA box. This promoter functions following DNA-mediated transfection into mammalian cells. The promoter of the mouse pgk-1 gene is homologous to the human pgk-1 promoter. A number of conserved motifs in the promoter may indicate a significant role for these sequences in expression of the pgk-1 gene. |
