| First Author | Chen HM | Year | 1998 |
| Journal | Gene | Volume | 207 |
| Issue | 2 | Pages | 209-18 |
| PubMed ID | 9511763 | Mgi Jnum | J:46100 |
| Mgi Id | MGI:1197131 | Doi | 10.1016/s0378-1119(97)00629-x |
| Citation | Chen HM, et al. (1998) Two promoters direct expression of the murine Spi-B gene, an Ets family transcription factor. Gene 207(2):209-18 |
| abstractText | Spi-B and PU.1 (Spi-1) comprise the most divergent subfamily of the Ets transcription factor family. Spi-B and PU.1 bind to similar DNA sequences, and can activate the same B-cell and myeloid promoters in vitro. However, PU.1 knockout mice demonstrate defective hematopoietic development of multiple hematopoietic lineages, indicating that Spi-B was not able to compensate for loss of PU.1. One explanation for these results is that, in contrast to PU.1, which is expressed in myeloid and B-cell lines, Spi-B expression is restricted to B-cells. In order to begin to understand the control of regulation of the Spi-B gene, murine Spi-B cDNA and genomic clones were isolated. The exon/intron organization and transcriptional start sites were determined; two major transcriptional start sites were detected. The two Spi-B promoters were isolated and characterized, and displayed differential activity in B-cell lines matching that of the endogenous gene. Further study of the two Spi-B promoters will provide insight into the molecular events regulating the tissue-specific and developmental stage-specific expression of Spi-B in B-cells. |
