| First Author | Inagaki N | Year | 1994 |
| Journal | J Biochem | Volume | 116 |
| Issue | 2 | Pages | 239-42 |
| PubMed ID | 7822236 | Mgi Jnum | J:20188 |
| Mgi Id | MGI:68298 | Doi | 10.1093/oxfordjournals.jbchem.a124512 |
| Citation | Inagaki N, et al. (1994) Cloning of a mouse Rabphilin-3A expressed in hormone-secreting cells. J Biochem 116(2):239-42 |
| abstractText | Rab3A, a ras p21-related small GTP-binding protein, is implicated in the exocytosis of neurotransmitters. Recently, Rabphilin-3A, a putative target protein for Rab3A, was identified and its cDNA has been cloned from bovine brain. In this study, we isolated a cDNA encoding a mouse Rabphilin-3A homolog from the insulin-secreting cell line, MIN6. Mouse Rabphilin-3A is a protein of 681 amino acids exhibiting overall 88.5% identity with bovine Rabphilin-3A. The amino acid identity between mouse and bovine Rabphilin-3A is highest in their carboxyl-terminal halves (97.8% identity) and amino-termini (93.0% identity), which contain the region of the two internal repeats homologous to the regulatory domain (C2 domain) of protein kinase C and the putative Rab3A-binding region, respectively. RNA blot analysis revealed that Rabphilin-3A mRNA is expressed in endocrine and hormone-secreting clonal cells, including rat adrenal glands, MIN6, the hamster insulin-secreting cell line, HIT-T15, and the rat catecholamine-secreting cell line, PC12, as well as rat brain. These results suggest that Rabphilin-3A might be involved in the exocytosis of secretory vesicles in hormone-secreting cells as well as in neurons. |
