| First Author | Wrammert J | Year | 2004 |
| Journal | Eur J Immunol | Volume | 34 |
| Issue | 1 | Pages | 137-46 |
| PubMed ID | 14971039 | Mgi Jnum | J:87667 |
| Mgi Id | MGI:3027402 | Doi | 10.1002/eji.200324225 |
| Citation | Wrammert J, et al. (2004) Identification of a novel thioredoxin-related protein, PC-TRP, which is preferentially expressed in plasma cells. Eur J Immunol 34(1):137-46 |
| abstractText | Using cDNA subtraction we have identified a previously unknown cDNA, termed plasma cell thioredoxin-related protein (PC-TRP), which was overexpressed in plasma cells in vitro. The full-length 2636-bp PC-TRP cDNA contained an open reading frame coding for 417 amino acids. The cDNA sequence was mapped to a region on mouse chromosome 13 and the PC-TRP gene consists of ten exons distributed over a 28-kb region. The PC-TRP was found to contain three atypical thioredoxin domains and showed functional thioredoxin activity in vitro. A more detailed analysis revealed that PC-TRP was expressed at a high level in plasma cells, both at the mRNA and protein level, while present at very low levels in all other cells and tissues examined. Immunohistological analysis revealed that PC-TRP was expressed at high levels in all plasma cells isolated from bone marrow or lamina propria, irrespective of their Ig isotype. In the spleen, however, 60% of the IgG-expressing plasma cells showed only a dull staining at day 14 after immunization, while >90% of the IgG-secreting cells in the spleen expressed PC-TRP at day 21 after immunization. In addition, an IgG-secreting, PC-TRPdull plasma cell population could also be observed after B cell activation in vitro. |
