| First Author | Giri PR | Year | 1991 |
| Journal | Biochem Biophys Res Commun | Volume | 181 |
| Issue | 1 | Pages | 252-8 |
| PubMed ID | 1659808 | Mgi Jnum | J:368 |
| Mgi Id | MGI:48906 | Doi | 10.1016/s0006-291x(05)81410-x |
| Citation | Giri PR, et al. (1991) Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit. Biochem Biophys Res Commun 181(1):252-8 |
| abstractText | Multiple catalytic subunits of the Ca2+ and calmodulin (CaM)-dependent protein phosphatase (PrP) (calcineurin or PrP-2B) are derived from at least two structural genes, type 1 (calcineurin A alpha) and type 2 (calcineurin A beta ), each of which can produce alternatively spliced transcripts. To examine the possible linkage of these genes, we analyzed genomic DNA from human/hamster hybrid cell lines using probes of 122 base pairs that were designed to bind selectively to exon 3 of the open reading frame. In this region, the nucleotide sequence of the type 2 murine cDNA that we cloned was greater than 99% identical to the type 2 human cDNA but only 78% identical to the type 1 human cDNA. Hybridization to Southern blots containing DNA from all human chromosomes showed that gene 1 was found on chromosome 4, whereas gene 2 segregated to chromosome 10. These data suggest that expression of the two calcineurin genes is not physically linked. |
