| First Author | Sok J | Year | 2001 |
| Journal | Cell Stress Chaperones | Volume | 6 |
| Issue | 1 | Pages | 6-15 |
| PubMed ID | 11525245 | Mgi Jnum | J:83417 |
| Mgi Id | MGI:2661479 | Doi | 10.1379/1466-1268(2001)006<0006:airapa>2.0.co;2 |
| Citation | Sok J, et al. (2001) Arsenite-inducible RNA-associated protein (AIRAP) protects cells from arsenite toxicity. Cell Stress Chaperones 6(1):6-15 |
| abstractText | Exposure of cells to arsenicals activates multiple stress pathways resulting in the induction of specific genes whose identity and role in the adaptation to arsenical-induced cellular stress are poorly understood. We report here the identification of a novel gene encoding an arsenite-inducible, cysteine- and histidine-rich RNA-associated protein, AIRAP, that is conserved among mammals, Drosophila and C elegans. Immunochemistry and cell fractionation experiments indicate that, when induced, AIRAP is present in both the nucleus and the cytoplasm, and cross-linking experiments indicate that it associates with RNA in vivo. The expression of a C elegans homologue of AIRAP, aip-1, is also induced by exposure to arsenite, and expression of an aip-1::gfp transgene is most pronounced in hypodermal cells. RNA-mediated interference (RNAi) of aip-1 lowers the resistance of nematodes to arsenite yet does not appear to affect viability under standard growth conditions. These experiments suggest a role for AIRAP/AIP-1 in protecting cells from the toxic effects of arsenite. |
