| First Author | Dooley TP | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 216 |
| Issue | 3 | Pages | 1101-9 |
| PubMed ID | 7488186 | Mgi Jnum | J:29862 |
| Mgi Id | MGI:77386 | Doi | 10.1006/bbrc.1995.2734 |
| Citation | Dooley TP, et al. (1995) Mouse rhodanese gene (Tst): cDNA cloning, sequencing, and recombinant protein expression. Biochem Biophys Res Commun 216(3):1101-9 |
| abstractText | Rhodanese (thiosulfate sulfurtransferase) is expressed at high levels in liver and is involved in the detoxification of cyanide. The full-length cDNA corresponding to the mouse rhodanese gene (Tst), which is located on chromosome 15, was cloned by PCR amplification of a liver cDNA library and subjected to DNA sequencing. Alignment of the rhodanese cDNA sequences from mouse and rat, which we previously cloned (Biochem. J. 275:227-231), revealed 97.3 percent identity at the protein level and 94.6 percent identity at the DNA level. When the mouse and rat cDNAs were expressed under the control of IPTG-inducible promoters in E. coli, the cell extracts exhibited cyanide-metabolizing activity, indicating that both genes encode functional rhodanese molecules. |
