| First Author | Nakayama K | Year | 1991 |
| Journal | J Biochem | Volume | 109 |
| Issue | 6 | Pages | 803-6 |
| PubMed ID | 1657897 | Mgi Jnum | J:33115 |
| Mgi Id | MGI:80596 | Doi | 10.1093/oxfordjournals.jbchem.a123461 |
| Citation | Nakayama K, et al. (1991) Cloning and functional expression of a novel endoprotease involved in prohormone processing at dibasic sites. J Biochem 109(6):803-6 |
| abstractText | We cloned and sequenced a cDNA from a library of mouse pituitary AtT-20 cells which are known to cleave an endogenous and various foreign prohormones at dibasic sites. This cDNA encodes a novel 753-residue protein, named PC3, which is structurally related to the yeast Kex2 protease involved in precursor cleavage at dibasic sites and to recently identified mammalian Kex2-like proteins, furin and PC2. Among examined cell lines and tissues, PC3 mRNA was only detected in AtT-20 cells. The substrate specificity of PC3 expressed in mammalian cells was similar to that observed in AtT-20 cells. We conclude that PC3 is a resident prohormone processing endoprotease in AtT-20 cells. |
