| First Author | Hsu DK | Year | 1996 |
| Journal | J Cell Physiol | Volume | 167 |
| Issue | 2 | Pages | 261-8 |
| PubMed ID | 8613466 | Mgi Jnum | J:32736 |
| Mgi Id | MGI:80224 | Doi | 10.1002/(SICI)1097-4652(199605)167:2<261::AID-JCP9>3.0.CO;2-N |
| Citation | Hsu DK, et al. (1996) Fibroblast growth factor-1-inducible gene FR-17 encodes a nonmuscle alpha-actinin isoform. J Cell Physiol 167(2):261-8 |
| abstractText | Polypeptide growth factor binding to cell surface receptors activates a cytoplasmic signaling cascade that ultimately promotes the expression of specific nuclear genes. As an approach to investigate the molecular mechanism of fibroblast growth factor (FGF)-1 mitogenic signaling, we have begun to identify and characterize FGF-1-inducible genes in murine NIH 3T3 cells. Here we report that one of these genes, termed FGF-regulated (FR)-17, is predicted to encode a nonmuscle isoform of alpha-actinin, an actin cross-linking protein found along microfilaments and in focal adhesion plaques. FGF-1 induction of alpha-actinin mRNA expression is first detectable at 2 h after mitogen addition and is dependent on the novo RNA and protein synthesis. Maximal alpha-actinin mRNA expression, corresponding to an approximately nineteenfold level of induction, is present after 12 h of FGF-1 stimulation. Western blot analysis indicated that FGF-1-stimulated cells also produce an increased amount of alpha-actinin protein. The FGF-1-related mitogen FGF-2, calf serum, several of the polypeptide growth factors present in serum, and the tumor promoter phorbol myristate acetate can also induce alpha-actinin mRNA expression. Finally, nonmuscle alpha-actinin mRNA is expressed in vivo in a tissue-specific manner, with relatively high levels detected in adult mouse intestine and kidney. These results indicate that nonmuscle alpha-actinin is a serum-, polypeptide growth factor-, and tumor promoter-inducible gene in mouse fibroblasts. |
