| First Author | Durkin ME | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 250 |
| Issue | 1 | Pages | 125-30 |
| PubMed ID | 9735344 | Mgi Jnum | J:49764 |
| Mgi Id | MGI:1278097 | Doi | 10.1006/bbrc.1998.9285 |
| Citation | Durkin ME, et al. (1998) Evaluation of Lama5 as a candidate for the mouse ragged (Ra) mutation. Biochem Biophys Res Commun 250(1):125-30 |
| abstractText | The laminin alpha 5 chain is a component of the basement membranes of many developing and adult tissues. The mouse laminin alpha 5 chain gene (Lama5) has been mapped close to the locus of the semidominant ragged (Ra) mutation on distal chromosome 2. The cause of the Ra mutation, which is usually lethal in the homozygous state, has not been determined. We have investigated whether a defect in Lama5 is responsible for the ragged mutation, using the Ra-J strain. No differences in the level of the laminin alpha 5 chain transcript were found in placental RNA from homozygous Ra-J mutant embryos compared to normal littermates. Antiserum raised against a recombinant laminin alpha 5 chain polypeptide stained the basement membranes of both normal and homozygous mutant embryos to a similar extent. More precise mapping of Lama5 on an interspecific Ra backcross indicated that Lama5 is proximal to the Ra locus. These results exclude Lama5 as a candidate gene for the Ra mutation. (C) 1998 Academic Press. |
