| First Author | Rocca-Serra J | Year | 1983 |
| Journal | EMBO J | Volume | 2 |
| Issue | 6 | Pages | 867-72 |
| PubMed ID | 6416834 | Mgi Jnum | J:7237 |
| Mgi Id | MGI:55708 | Doi | 10.1002/j.1460-2075.1983.tb01515.x |
| Citation | Rocca-Serra J, et al. (1983) Analysis of antibody diversity: V-D-J mRNA nucleotide sequence of four anti-GAT monoclonal antibodies. A paucigene system using alternate D-J recombinations to generate functionally similar hypervariable regions. EMBO J 2(6):867-72 |
| abstractText | The nucleotide sequence of four anti-(Glu60-Ala30-Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2-terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These sequences, which are highly conserved, indicate that anti-GAT antibodies expressing the same public idiotypic specificities represent a paucigene system, which uses at least two D-J combinations leading to functionally similar hypervariable regions involved in the recognition of the dominant Glu-Tyr determinant. D regions are encoded by D genes which are closely related either to the D-SP2 or the D.FL16 germ line gene cores. |
