| First Author | Lerner A | Year | 1992 |
| Journal | Eur J Immunol | Volume | 22 |
| Issue | 8 | Pages | 2135-40 |
| PubMed ID | 1322304 | Mgi Jnum | J:2061 |
| Mgi Id | MGI:50585 | Doi | 10.1002/eji.1830220826 |
| Citation | Lerner A, et al. (1992) Human genomic sequences corresponding to murine CD3 eta-related transcripts: lack of conservation or expression of homologous human products. Eur J Immunol 22(8):2135-40 |
| abstractText | We have cloned and sequenced human genomic DNA homologous to exons 9 and 10 of the CD3 zeta/eta/theta locus. Although there are open reading frames within the human sequences corresponding to the translated portions of murine exons 9 and 10, we find no evidence of conservation of the encoded polypeptide product. Furthermore, using oligonucleotides derived from these homologous sequences, we are unable to detect human CD3 eta- or CD3 theta-like transcripts by polymerase chain reaction amplification of reverse-transcribed RNA from a variety of human lymphoid tissues. Despite the absence of evidence for conservation of human CD3 eta and CD3 theta, there is a surprising degree of similarity between human and murine nucleotide sequences, not only for exons 9 and 10 (78% and 70%, respectively), but also for the 9/10 intron (71%). A possible mechanism for this conservation is discussed. |
