| First Author | Radhakrishnan I | Year | 1997 |
| Journal | Cell | Volume | 91 |
| Issue | 6 | Pages | 741-52 |
| PubMed ID | 9413984 | Mgi Jnum | J:240310 |
| Mgi Id | MGI:5882920 | Doi | 10.1016/s0092-8674(00)80463-8 |
| Citation | Radhakrishnan I, et al. (1997) Solution structure of the KIX domain of CBP bound to the transactivation domain of CREB: a model for activator:coactivator interactions. Cell 91(6):741-52 |
| abstractText | The nuclear factor CREB activates transcription of target genes in part through direct interactions with the KIX domain of the coactivator CBP in a phosphorylation-dependent manner. The solution structure of the complex formed by the phosphorylated kinase-inducible domain (pKID) of CREB with KIX reveals that pKID undergoes a coil-->helix folding transition upon binding to KIX, forming two alpha helices. The amphipathic helix alphaB of pKID interacts with a hydrophobic groove defined by helices alpha1 and alpha3 of KIX. The other pKID helix, alphaA, contacts a different face of the alpha3 helix. The phosphate group of the critical phosphoserine residue of pKID forms a hydrogen bond to the side chain of Tyr-658 of KIX. The structure provides a model for interactions between other transactivation domains and their targets. |
