| First Author | Carvalhal S | Year | 2015 |
| Journal | Mol Biol Cell | Volume | 26 |
| Issue | 19 | Pages | 3424-38 |
| PubMed ID | 26246606 | Mgi Jnum | J:273545 |
| Mgi Id | MGI:6294217 | Doi | 10.1091/mbc.E15-02-0113 |
| Citation | Carvalhal S, et al. (2015) The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation. Mol Biol Cell 26(19):3424-38 |
| abstractText | The formation of the mitotic spindle is a complex process that requires massive cellular reorganization. Regulation by mitotic kinases controls this entire process. One of these mitotic controllers is Aurora A kinase, which is itself highly regulated. In this study, we show that the nuclear pore protein ALADIN is a novel spatial regulator of Aurora A. Without ALADIN, Aurora A spreads from centrosomes onto spindle microtubules, which affects the distribution of a subset of microtubule regulators and slows spindle assembly and chromosome alignment. ALADIN interacts with inactive Aurora A and is recruited to the spindle pole after Aurora A inhibition. Of interest, mutations in ALADIN cause triple A syndrome. We find that some of the mitotic phenotypes that we observe after ALADIN depletion also occur in cells from triple A syndrome patients, which raises the possibility that mitotic errors may underlie part of the etiology of this syndrome. |
