| First Author | Johnsen LB | Year | 1995 |
| Journal | Biochem J | Volume | 309 ( Pt 1) |
| Pages | 237-42 | PubMed ID | 7619062 |
| Mgi Jnum | J:26727 | Mgi Id | MGI:74159 |
| Doi | 10.1042/bj3090237 | Citation | Johnsen LB, et al. (1995) Characterization of three types of human alpha s1-casein mRNA transcripts. Biochem J 309(Pt 1):237-42 |
| abstractText | Here we report the molecular cloning and sequencing of three types of human alpha s1-casein transcripts and present evidence indicating that exon skipping is responsible for deleted mRNA transcripts. The largest transcript comprised 981 bp encoding a signal peptide of 15 amino acids followed by the mature alpha s1-casein sequence of 170 amino acids. Human alpha s1-casein has been reported to exist naturally as a multimer in complex with kappa-casein in mature human milk, thereby being unique among alpha s1-caseins [Rasmussen, Due and Petersen (1995) Comp. Biochem. Physiol., in the press]. The present demonstration of three cysteines in the mature protein provides a molecular explanation of the interactions in this complex. Tissue-specific expression of human alpha s1-casein was indicated by Northern-blot analysis. In addition, two cryptic exons were localized in the bovine alpha s1-casein gene. |
