| First Author | Tran C | Year | 2015 |
| Journal | Life Sci | Volume | 121 |
| Pages | 35-9 | PubMed ID | 25476831 |
| Mgi Jnum | J:244894 | Mgi Id | MGI:5913673 |
| Doi | 10.1016/j.lfs.2014.11.017 | Citation | Tran C, et al. (2015) Role of caveolin-3 in lymphocyte activation. Life Sci 121:35-9 |
| abstractText | AIMS: Caveolins are structural proteins clustered in lipid-rich regions of plasma membrane involved in coordinating signal transduction in various organ systems. While caveolin-1 (Cav-1) has been shown to regulate lymphocyte activation, the role of caveolin-3 (Cav-3) in immune system signaling has not been investigated. We tested the hypothesis that Cav-3 modulates lymphocyte activation. MAIN METHODS: Lymphocyte/leukocyte subpopulations from WT and Cav-3 mice were profiled with flow cytometry. Cytokine production in quiescent and activated splenocytes from WT and Cav-3 mice was assessed with ELISA. KEY FINDINGS: Levels of T-cells, monocytes, and natural killer cells were not different between WT and KO mice, however KO mice had lower B-cell population-percentage. Functionally, activated lymphocytes from Cav-3 KO mice demonstrated significantly reduced expression of IL-2 compared to WT, while expression of TNFalpha, IL-6, and IL-10 was not different. Finally, expression of IL-17 was significantly reduced in T-helper cells from KO mice, while IFNgamma was not, suggesting that Cav-3 is a determinant in the development of the Th-17 subpopulation. SIGNIFICANCE: This study is the first to demonstrate that Cav-3 may be a novel participant in B-cell expression, T-cell cytokine production and activation of inflammation. |
