| Primary Identifier | IPR004881 | Type | Family |
| Short Name | Ribosome_biogen_GTPase_RsgA |
| description | This entry contains Escherichia coli (strain K12) RsgA, which plays a role in the late maturation steps of the functional core of the 30S ribosomal subunit. It removes RbfA from mature, but not immature, 30S ribosomes in a GTP-dependent manner [, ], and binds the 30S subunit making contact with the head, platform and rRNA helix 44 [, ]. RsgA (sometimes known as YjeQ or EngC) is an unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover. It is dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96 fold increase in kcat) ribosomes []. The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin []streptomycin and spectinomycin []. This inhibition is not due to competition for binding sites on the 30S or 70S ribosome []. A mitochondrial version of RsgA has been characterized from Arabidopsis[]. |
