| Primary Identifier | IPR012708 | Type | Family |
| Short Name | 2Me_IsoCit_deHydtase_FeS-dep |
| description | This entry represents Fe/S-dependent 2-methylisocitrate dehydratase (AcnD; ), which is part of the 2-methylcitrate (2-MC) cycle that occurs in certain fungi and bacteria. The 2-MC cycle is involved in the degradation of propionyl-CoA via 2-methylcitrate, with AcnD functioning after PrpD and before PrpB. AcnD acts to catalyse the dehydration of 2-methylcitrate and citrate to 2-methyl-cis-aconitate and cis-aconitate, respectively, as well as to catalyse the hydration of cis-aconitate. However, 2-methylisocitrate and isocitrate were not substrates for AcnD, indicating that AcnD only catalyses the first half of the aconitase-like dehydration reactions []. The enzyme from the fungus Yarrowia lipolytica (Candida lipolytica) does not act on isocitrate. AcnD is homologous to aconitases A and B. In Escherichia coli, which lacks a member of this family, 2-methylisocitrate dehydratase activity was traced to aconitase B () []. |
