| Primary Identifier | IPR015186 | Type | Domain |
| Short Name | Exotox-A_middle_dom |
| description | This domain, found in Pseudomonas aeruginosa exotoxin A, is responsible for transmembrane targeting of the toxin, as well as transmembrane translocation of the catalytic domain into the cytoplasmic compartment. A furin cleavage site is present within the domain: cleavage generates a 37kDa carboxy-terminal fragment, which includes the enzymatic domain, which is then is translocated into the cytoplasm. It adopts a helical structure, with six α-helices forming a bundle []. Proteins contain this domain include exotoxin A from Pseudomonas aeruginosa and cholix toxin from Vibrio cholerae []. They are NAD-dependent ADP-ribosyltransferases (ADPRT) that catalyses the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis []. |
