| Primary Identifier | IPR037038 | Type | Homologous_superfamily |
| Short Name | HepT-like_sf |
| description | This entry describes prokaryotic proteins including tRNA nuclease HepT from Aphanizomenon flos-aquae, the toxic component of a type II toxin-antitoxin (TA) system comprising a minimal nucleotidyltransferase (MNT) and the accompanying HEPN (higher eukaryotes and prokaryotes nucleotide-binding) protein []. HepT dimerises and enables the formation of a deep cleft at the HEPN-domain interface, containing the RX4-6H motif (where X is any amino acid and the residue immediately after the conserved R is typically a polar amino acid), the active site that functions as an RNA-cleaving RNase []. HepT from Aphanizomenon flos-aquae targets a subset of tRNAs and cuts off 4 nt from the 3' end of tRNA acceptor stem, but its toxicity is neutralized by covalent di-AMPylation performed by the MNT antitoxin []. |
