| Primary Identifier | IPR007807 | Type | Domain |
| Short Name | TcmA/NAT10_helicase |
| description | This domain contains a P-loop (Walker A) motif, suggesting that it has ATPase activity, and a Walker B motif. It is found in NAT10 from eukaryotes and TmcA from prokaryotes. In NAT10, this domain catalyses the formation of N4-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs [, , ]. In TmcA, it may function as an RNA helicase motor (driven by ATP hydrolysis) which delivers the wobble base to the active centre of the GCN5-related N-acetyltransferase (GNAT) domain [].The bacterial tRNA(Met) cytidine acetyltransferase (TmcA) catalyses the formation of N(4)-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and either ATP or GTP []. This modification is thought to ensure precise recognition of the AUG codon by strengthening C-G base-pair interaction and also prevent misrecognition of the near cognate AUA codon []. RNA cytidine acetyltransferas (NAT10) are RNA cytosine acetyltransferases with specificity toward both 18S rRNA and tRNAs and contain additional putative nuclear and nucleolar localisation signals (NLS and NoLS respectively) [, , ]. |
