| First Author | Al-Qusairi L | Year | 2017 |
| Journal | J Am Soc Nephrol | Volume | 28 |
| Issue | 8 | Pages | 2431-2442 |
| PubMed ID | 28289184 | Mgi Jnum | J:290403 |
| Mgi Id | MGI:6436203 | Doi | 10.1681/ASN.2016070732 |
| Citation | Al-Qusairi L, et al. (2017) Renal Tubular Ubiquitin-Protein Ligase NEDD4-2 Is Required for Renal Adaptation during Long-Term Potassium Depletion. J Am Soc Nephrol 28(8):2431-2442 |
| abstractText | Adaptation of the organism to potassium (K(+)) deficiency requires precise coordination among organs involved in K(+) homeostasis, including muscle, liver, and kidney. How the latter performs functional and molecular changes to ensure K(+) retention is not well understood. Here, we investigated the role of ubiquitin-protein ligase NEDD4-2, which negatively regulates the epithelial sodium channel (ENaC), Na(+)/Cl(-) cotransporter (NCC), and with no-lysine-kinase 1 (WNK1). After dietary K(+) restriction for 2 weeks, compared with control littermates, inducible renal tubular NEDD4-2 knockout (Nedd4L(Pax8/LC1) ) mice exhibited severe hypokalemia and urinary K(+) wasting. Notably, expression of the ROMK K(+) channel did not change in the distal convoluted tubule and decreased slightly in the cortical/medullary collecting duct, whereas BK channel abundance increased in principal cells of the connecting tubule/collecting ducts. However, K(+) restriction also enhanced ENaC expression in Nedd4L(Pax8/LC1) mice, and treatment with the ENaC inhibitor, benzamil, reversed excessive K(+) wasting. Moreover, K(+) restriction increased WNK1 and WNK4 expression and enhanced SPAK-mediated NCC phosphorylation in Nedd4L(Pax8/LC1) mice, with no change in total NCC. We propose a mechanism in which NEDD4-2 deficiency exacerbates hypokalemia during dietary K(+) restriction primarily through direct upregulation of ENaC, whereas increased BK channel expression has a less significant role. These changes outweigh the compensatory antikaliuretic effects of diminished ROMK expression, increased NCC phosphorylation, and enhanced WNK pathway activity in the distal convoluted tubule. Thus, NEDD4-2 has a crucial role in K(+) conservation through direct and indirect effects on ENaC, distal nephron K(+) channels, and WNK signaling. |
