| First Author | Liu S | Year | 2013 |
| Journal | J Invest Dermatol | Volume | 133 |
| Issue | 4 | Pages | 899-906 |
| PubMed ID | 23190902 | Mgi Jnum | J:196513 |
| Mgi Id | MGI:5488675 | Doi | 10.1038/jid.2012.438 |
| Citation | Liu S, et al. (2013) Integrin beta1 is required for dermal homeostasis. J Invest Dermatol 133(4):899-906 |
| abstractText | Integrins are heterodimers that mediate cell attachment to the extracellular matrix. Previously, we used fibroblast-specific knockout mice to show that the integrin beta1 subunit was required in vivo for dermal fibrogenesis and cutaneous tissue repair. Here, we show that integrin beta1 expression by fibroblasts is required for dermal homeostasis; at 8 weeks after deletion of integrin beta1, the dermis is significantly thinner, expressing less collagen and displaying reduced generation of reactive oxygen species (ROS). Cultured integrin beta1-deficient fibroblasts show reduced rac1 activation and ROS generation. Overexpressing rac1 in integrin beta1-deficient fibroblasts restored ROS generation; adding hydrogen peroxide to integrin beta1-deficient fibroblasts restored Col1a2 (collagen, type I, alpha2) and alpha-smooth muscle actin (alpha-SMA) mRNA expression and alpha-SMA protein expression and stress fiber formation. Thus integrin beta1 expression by fibroblasts is required for the maintenance of dermal connective tissue via a rac/ROS pathway. |
