| First Author | Ruf S | Year | 2011 |
| Journal | Nat Genet | Volume | 43 |
| Issue | 4 | Pages | 379-86 |
| PubMed ID | 21423180 | Mgi Jnum | J:180121 |
| Mgi Id | MGI:5305446 | Doi | 10.1038/ng.790 |
| Citation | Ruf S, et al. (2011) Large-scale analysis of the regulatory architecture of the mouse genome with a transposon-associated sensor. Nat Genet 43(4):379-86 |
| abstractText | We present here a Sleeping Beauty-based transposition system that offers a simple and efficient way to investigate the regulatory architecture of mammalian chromosomes in vivo. With this system, we generated several hundred mice and embryos, each with a regulatory sensor inserted at a random genomic position. This large sampling of the genome revealed the widespread presence of long-range regulatory activities along chromosomes, forming overlapping blocks with distinct tissue-specific expression potentials. The presence of tissue-restricted regulatory activities around genes with widespread expression patterns challenges the gene-centric view of genome regulation and suggests that most genes are modulated in a tissue-specific manner. The local hopping property of Sleeping Beauty provides a dynamic approach to map these regulatory domains at high resolution and, combined with Cre-mediated recombination, allows for the determination of their functions by engineering mice with specific chromosomal rearrangements. |
