| Primary Identifier | MGI:2655527 | Allele Type | Targeted |
| Attribute String | Conditional ready, Modified isoform(s) | Gene | Fn1 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The endogenous EDA exon, which is subject to alternative splicing, was replaced with a floxed EDA exon modified with point mutations for optimization of both the 5' and the 3' splice sites. RNase protection and RT-PCR analysis confirmed constitutive EDA exon inclusion, showing the EDA exon to be included in 99-100% of transcript in all tissues of homozygous mutant mice. |
