| First Author | Gundry MC | Year | 2016 |
| Journal | Cell Rep | Volume | 17 |
| Issue | 5 | Pages | 1453-1461 |
| PubMed ID | 27783956 | Mgi Jnum | J:240901 |
| Mgi Id | MGI:5896706 | Doi | 10.1016/j.celrep.2016.09.092 |
| Citation | Gundry MC, et al. (2016) Highly Efficient Genome Editing of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9. Cell Rep 17(5):1453-1461 |
| abstractText | Our understanding of the mechanisms that regulate hematopoietic stem/progenitor cells (HSPCs) has been advanced by the ability to genetically manipulate mice; however, germline modification is time consuming and expensive. Here, we describe fast, efficient, and cost-effective methods to directly modify the genomes of mouse and human HSPCs using the CRISPR/Cas9 system. Using plasmid and virus-free delivery of guide RNAs alone into Cas9-expressing HSPCs or Cas9-guide RNA ribonucleoprotein (RNP) complexes into wild-type cells, we have achieved extremely efficient gene disruption in primary HSPCs from mouse (>60%) and human ( approximately 75%). These techniques enabled rapid evaluation of the functional effects of gene loss of Eed, Suz12, and DNMT3A. We also achieved homology-directed repair in primary human HSPCs (>20%). These methods will significantly expand applications for CRISPR/Cas9 technologies for studying normal and malignant hematopoiesis. |
