| First Author | Li J | Year | 2018 |
| Journal | J Cell Biol | Volume | 217 |
| Issue | 11 | Pages | 3901-3911 |
| PubMed ID | 30097513 | Mgi Jnum | J:267475 |
| Mgi Id | MGI:6256702 | Doi | 10.1083/jcb.201802077 |
| Citation | Li J, et al. (2018) Cyclin B2 can compensate for Cyclin B1 in oocyte meiosis I. J Cell Biol 217(11):3901-3911 |
| abstractText | Mammalian oocytes are arrested at the prophase of the first meiotic division for months and even years, depending on species. Meiotic resumption of fully grown oocytes requires activation of M-phase-promoting factor (MPF), which is composed of Cyclin B1 and cyclin-dependent kinase 1 (CDK1). It has long been believed that Cyclin B1 synthesis/accumulation and its interaction with CDK1 is a prerequisite for MPF activation in oocytes. In this study, we revealed that oocyte meiotic resumption occurred in the absence of Cyclin B1. Ccnb1-null oocytes resumed meiosis and extruded the first polar body. Without Cyclin B1, CDK1 could be activated by up-regulated Cyclin B2. Ccnb1 and Ccnb2 double knockout permanently arrested the oocytes at the prophase of the first meiotic division. Oocyte-specific Ccnb1-null female mice were infertile due to failed MPF activity elevation and thus premature interphase-like stage entry in the second meiotic division. These results have revealed a hidden compensatory mechanism between Cyclin B1 and Cyclin B2 in regulating MPF and oocyte meiotic resumption. |
