| Primary Identifier | MGI:2386961 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Plxna3 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 2-4 were replaced with a GFP-neo cassette via homologous recombination. The gene targeting event deletes the first 440 amino acids of the protein, which encodes the entire signal sequence and most of the extracellular Sema-like domain. Absence of gene expression in homozygous mutants was confirmed by RT-PCR analysis of RNA from E16.5 embryos and by Western analysis of brain lysates from postnatal day 1 homozygous mutant neonates. |
