| First Author | Shimizu R | Year | 2001 |
| Journal | EMBO J | Volume | 20 |
| Issue | 18 | Pages | 5250-60 |
| PubMed ID | 11566888 | Mgi Jnum | J:71847 |
| Mgi Id | MGI:2150878 | Doi | 10.1093/emboj/20.18.5250 |
| Citation | Shimizu R, et al. (2001) In vivo requirements for GATA-1 functional domains during primitive and definitive erythropoiesis. EMBO J 20(18):5250-60 |
| abstractText | GATA-1 is a transcription factor essential for erythroid/megakaryocytic cell differentiation. To investigate the contribution of individual domains of GATA-1 to its activity, transgenic mice expressing either an N-terminus, or an N- or C-terminal zinc finger deletion of GATA-1 (Delta NT, Delta NF or Delta CF, respectively) were generated and crossed to GATA-1 germline mutant (GATA-1.05) mice. Since the GATA-1 gene is located on the X-chromosome, male GATA-1 mutants die by embryonic day 12.5. Both Delta NF and Delta CF transgenes failed to rescue the GATA-1.05/Y pups. However, transgenic mice expressing Delta NT, but not the Delta NF protein, were able to rescue definitive hematopoiesis. In embryos, while neither the Delta CF protein nor a mutant missing both N-terminal domains (Delta NTNF) was able to support primitive erythropoiesis, the two independent Delta NT and Delta NF mutants could support primitive erythropoiesis. Thus, lineage-specific transgenic rescue of the GATA-1 mutant mouse revealed novel properties that are conferred by specific domains of GATA-1 during primitive and definitive erythropoiesis, and demonstrate that the NT and NF moieties lend complementary, but distinguishable properties to the function of GATA-1. |
