| Primary Identifier | MGI:2677488 | Allele Type | Targeted |
| Gene | Nkx2-1 | Transmission | Germline |
| Strain of Origin | 129S2/SvPas | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell line = MPI1 (MPI-I). |
| molecularNote | Sequence encoding S80, a phosphorylation mutant allele of the orthologous rat gene in which 7 serine codons are replaced with alanine codons, was inserted in place of a fragment extending from the start codon (bp 7957) to the end of the homeobox (bp 9480). A polyadenylation signal and neo cassette were included immediately downstream of S80. Southern blot analysis confirmed the integration of the mutation that putatively produces a protein lacking residues required for phosphorylation. |
