| First Author | Dong TX | Year | 2017 |
| Journal | Elife | Volume | 6 |
| PubMed ID | 29239725 | Mgi Jnum | J:254963 |
| Mgi Id | MGI:6110904 | Doi | 10.7554/eLife.32417 |
| Citation | Dong TX, et al. (2017) T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse. Elife 6:e32417 |
| abstractText | Calcium is an essential cellular messenger that regulates numerous functions in living organisms. Here, we describe development and characterization of ''Salsa6f'', a fusion of GCaMP6f and tdTomato optimized for cell tracking while monitoring cytosolic Ca(2+), and a transgenic Ca(2+) reporter mouse with Salsa6f targeted to the Rosa26 locus for Cre-dependent expression in specific cell types. The development and function of T cells was unaffected in Cd4-Salsa6f mice. We describe Ca(2+) signals reported by Salsa6f during T cell receptor activation in naive T cells, helper Th17 T cells and regulatory T cells, and Ca(2+) signals mediated in T cells by an activator of mechanosensitive Piezo1 channels. Transgenic expression of Salsa6f enables ratiometric imaging of Ca(2+) signals in complex tissue environments found in vivo. Two-photon imaging of migrating T cells in the steady-state lymph node revealed both cell-wide and localized sub-cellular Ca(2+) transients (''sparkles'') as cells migrate. |
