| First Author | Liu H | Year | 2016 |
| Journal | Sci Rep | Volume | 6 |
| Pages | 22040 | PubMed ID | 26907705 |
| Mgi Jnum | J:338009 | Mgi Id | MGI:6219634 |
| Doi | 10.1038/srep22040 | Citation | Liu H, et al. (2016) TLR5 mediates CD172alpha(+) intestinal lamina propria dendritic cell induction of Th17 cells. Sci Rep 6:22040 |
| abstractText | Multiple mechanisms exist in regulation of host responses to massive challenges from microbiota to maintain immune homeostasis in the intestines. Among these is the enriched Th17 cells in the intestines, which regulates intestinal homeostasis through induction of antimicrobial peptides and secretory IgA among others. However, the means by which Th17 cells develop in response to microbiota is still not completely understood. Although both TLR5 and CD172alpha(+) lamina propria dendritic cells (LPDC) have been shown to promote Th17 cell development, it is still unclear whether TLR5 mediates the CD172alpha(+)LPDC induction of Th17 cells. By using a microbiota antigen-specific T cell reporter mouse system, we demonstrated that microbiota antigen-specific T cells developed into Th17 cells in the intestinal LP, but not in the spleen when transferred into TCRbetaxdelta(-/-) mice. LPDCs expressed high levels of TLR5, and most CD172alpha(+)LPDCs also co-expressed TLR5. LPDCs produced high levels of IL-23, IL-6 and TGFbeta when stimulated with commensal flagellin and promoted Th17 cell development when cultured with full-length CBir1 flagellin but not CBir1 peptide. Wild-type CD172alpha(+), but not CD172alpha(-), LPDCs induced Th17 cells, whereas TLR5-deficient LPDC did not induce Th17 cells. Our data thereby demonstrated that TLR5 mediates CD172alpha(+)LPDC induction of Th17 cells in the intestines. |
