| First Author | Takenaka E | Year | 2018 |
| Journal | Sci Rep | Volume | 8 |
| Issue | 1 | Pages | 15180 |
| PubMed ID | 30315271 | Mgi Jnum | J:268098 |
| Mgi Id | MGI:6270697 | Doi | 10.1038/s41598-018-33437-4 |
| Citation | Takenaka E, et al. (2018) Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4(+) T cell costimulation. Sci Rep 8(1):15180 |
| abstractText | Mouse peritoneal macrophages consist of two subsets: large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs), defined as CD11b(hi)F4/80(hi) and CD11b(+)F4/80(lo) cells, respectively. We reveal that SPMs, but not LPMs, have the ability to present antigens to naive CD4(+) T cells. Coculture of SPMs with naive ovalbumin (OVA) specific CD4(+) T cells (OT-II) in the presence of OVA peptide effectively induced CD4(+) T cells priming. SPMs, but not LPMs, strongly express DNAM-1, an activating immunoreceptor. Although antigen uptake and processing were comparable between WT and DNAM-1-deficient SPMs, deficiency of DNAM-1 on SPMs or blockade of DNAM-1 and its ligand interaction impaired CD4(+) T cells priming by SPMs. Furthermore, T and B cell responses in mediastinal lymph nodes of mice intraperitoneally immunized with trinitrophenyl (TNP)-OVA protein in Alum adjuvant were enhanced by intraperitoneally transferred wild-type, but not DNAM-1-deficient, SPMs. We propose that SPMs are functionally distinct from LPMs, and DNAM-1 plays a costimulatory role in antigen presentation by SPMs. |
