| First Author | Gray LT | Year | 2017 |
| Journal | Elife | Volume | 6 |
| PubMed ID | 28112643 | Mgi Jnum | J:253162 |
| Mgi Id | MGI:6108385 | Doi | 10.7554/eLife.21883 |
| Citation | Gray LT, et al. (2017) Layer-specific chromatin accessibility landscapes reveal regulatory networks in adult mouse visual cortex. Elife 6:e21883 |
| abstractText | Mammalian cortex is a laminar structure, with each layer composed of a characteristic set of cell types with different morphological, electrophysiological, and connectional properties. Here, we define chromatin accessibility landscapes of major, layer-specific excitatory classes of neurons, and compare them to each other and to inhibitory cortical neurons using the Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq). We identify a large number of layer-specific accessible sites, and significant association with genes that are expressed in specific cortical layers. Integration of these data with layer-specific transcriptomic profiles and transcription factor binding motifs enabled us to construct a regulatory network revealing potential key layer-specific regulators, including Cux1/2, Foxp2, Nfia, Pou3f2, and Rorb. This dataset is a valuable resource for identifying candidate layer-specific cis-regulatory elements in adult mouse cortex. |
